SMC3 antibody (N-Term)

Catalog No. ABIN7466888

This anti-SMC3 antibody is a Rabbit Polyclonal antibody detecting SMC3 in WB, IP and IHC (p). Suitable for Human.

Quick Overview for SMC3 antibody (N-Term) (ABIN7466888)

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMC3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SMC3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse liver , A431 , H1299 , HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SMC3

Target: SMC3 (Structural Maintenance of Chromosomes 3 (SMC3))

Alternative Name: structural maintenance of chromosomes 3

Background: Structural maintenance of chromosomes 3 , BAM , BMH , CDLS3 , CSPG6 , HCAP , SMC3L1,This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq]

Molecular Weight: 142 kDa

Gene ID: 9126

UniProt: Q9UQE7

Pathways: Stem Cell Maintenance