BRISC and BRCA1 A Complex Member 1 (BABAM1) (N-Term) antibody

Catalog No. ABIN7467205

The Mouse Monoclonal anti- antibody has been validated for WB, IF and ICC. It is suitable to detect in samples from Human.

Quick Overview for BRISC and BRCA1 A Complex Member 1 (BABAM1) (N-Term) antibody (ABIN7467205)

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT1251

Product Details

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human HSPC142. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: BRISC and BRCA1 A Complex Member 1 (BABAM1)

Alternative Name: BRISC and BRCA1 A complex member 1

Background: BRISC and BRCA1 A complex member 1 , C19orf62 , HSPC142 , MERIT40 , NBA1,Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.

Molecular Weight: 37 kDa

Gene ID: 29086

UniProt: Q9NWV8

Pathways: Positive Regulation of Response to DNA Damage Stimulus