Cyclin D2 antibody (N-Term)

Catalog No. ABIN7467394

The Rabbit Polyclonal anti-Cyclin D2 antibody has been validated for WB, IF, ICC and IHC (p). It is suitable to detect Cyclin D2 in samples from Human.

Quick Overview for Cyclin D2 antibody (N-Term) (ABIN7467394)

Target: Cyclin D2 (CCND2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin D2 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cyclin D2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: RD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.97 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Cyclin D2

Target: Cyclin D2 (CCND2)

Alternative Name: cyclin D2

Background: Cyclin D2 , KIAK0002 , MPPH3,The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. [provided by RefSeq]

Molecular Weight: 33 kDa

Gene ID: 894

UniProt: P30279

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases