EIF5 antibody

Catalog No. ABIN7467577

The Rabbit Polyclonal anti-EIF5 antibody has been validated for WB and IHC (p). It is suitable to detect EIF5 in samples from Human.

Quick Overview for EIF5 antibody (ABIN7467577)

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF5 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: eukaryotic translation initiation factor 5

Background: Eukaryotic translation initiation factor 5 , EIF-5 , EIF-5A,Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM]

Molecular Weight: 49 kDa

Gene ID: 1983

UniProt: P55010