SKA1 antibody

Catalog No. ABIN7467814

The Rabbit Polyclonal anti-SKA1 antibody is suitable to detect SKA1 in samples from Human. It has been validated for IHC (p).

Quick Overview for SKA1 antibody (ABIN7467814)

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SKA1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SKA1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SKA1

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Alternative Name: spindle and kinetochore associated complex subunit 1

Background: Spindle and kinetochore associated complex subunit 1 , C18orf24,Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

Molecular Weight: 29 kDa

Gene ID: 220134

UniProt: Q96BD8

Pathways: M Phase