EHMT2 antibody

Catalog No. ABIN7467954

This Rabbit Polyclonal antibody specifically detects EHMT2 in WB, IF, IHC (p), ICC, IP and ChIP. It exhibits reactivity toward Human.

Quick Overview for EHMT2 antibody (ABIN7467954)

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Product Details anti-EHMT2 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human G9a. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.34 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: euchromatic histone lysine methyltransferase 2

Background:

Synonyms: euchromatic histone lysine methyltransferase 2 , BAT8 , C6orf30 , G9A , GAT8 , KMT1C , NG36

Background: This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Molecular Weight: 132 kDa

Gene ID: 10919

UniProt: Q96KQ7