BRCA2 antibody (AA 2336-2478)

Catalog No. ABIN7468065

This Mouse Monoclonal antibody specifically detects BRCA2 in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for BRCA2 antibody (AA 2336-2478) (ABIN7468065)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5F6

Product Details anti-BRCA2 Antibody

Binding Specificity: AA 2336-2478

Cross-Reactivity: Human

Purification: Protein G purified

Immunogen: Amino acids 2336-2478 of human BRCA2 expressed in E. coli. BRCA2-P400.

Isotype: IgG1

Application Details

Application Notes: Do not boil samples for longer than two minutes. For WB: Use at a concentration of 1-5 μg/mL. Predicted molecular weight: 380 kDa. Optimal dilutions/concentrations should be determined by the reseracher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2 DNA repair associated

Background:

Synonyms: BRCA2 DNA repair associated , BRCC2 , BROVCA2 , FACD , FAD , FAD1 , FANCD , FANCD1 , GLM3 , PNCA2 , XRCC11

Background: Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008]

Molecular Weight: 384 kDa

Gene ID: 675

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location