CAPG antibody

Catalog No. ABIN7468095

This Rabbit Polyclonal antibody specifically detects CAPG in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CAPG antibody (ABIN7468095)

Target: CAPG (Capping Protein (Actin Filament), Gelsolin-Like (CAPG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPG antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-CAPG Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CAPG. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , HeLa Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CAPG

Target: CAPG (Capping Protein (Actin Filament), Gelsolin-Like (CAPG))

Alternative Name: capping actin protein, gelsolin like

Background:

Synonyms: capping actin protein, gelsolin like , AFCP , HEL-S-66 , MCP

Background: This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Molecular Weight: 38 kDa

Gene ID: 822

UniProt: P40121

Pathways: Regulation of Actin Filament Polymerization