CD3EAP antibody

Catalog No. ABIN7468113

This Mouse Monoclonal antibody specifically detects CD3EAP in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CD3EAP antibody (ABIN7468113)

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3EAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: GT635

Product Details anti-CD3EAP Antibody

Cross-Reactivity: Human

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , A549 , H1299 , HCT116 Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CD3EAP

Target: CD3EAP (CD3e Molecule, epsilon Associated Protein (CD3EAP))

Alternative Name: CD3e molecule associated protein

Background:

Synonyms: CD3e molecule associated protein , ASE-1 , ASE1 , CAST , PAF49

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Molecular Weight: 55 kDa

Gene ID: 10849

UniProt: O15446