CDC6 antibody

Catalog No. ABIN7468126

The Rabbit Polyclonal anti-CDC6 antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect CDC6 in samples from Human.

Quick Overview for CDC6 antibody (ABIN7468126)

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDC6 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CDC6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , GL261 , C8D30 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , Jurkat , HeLa (10 mM Hydroxyurea treatment for 18 hr) Validation: Comparison, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.36 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CDC6

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Alternative Name: cell division cycle 6

Background:

Synonyms: cell division cycle 6 , CDC18L , HsCDC18 , HsCDC6 , MGORS5

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq]

Molecular Weight: 63 kDa

Gene ID: 990

UniProt: Q99741

Pathways: DNA Replication, Synthesis of DNA