DDX20 antibody

Catalog No. ABIN7468215

This Rabbit Polyclonal antibody specifically detects DDX20 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DDX20 antibody (ABIN7468215)

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DDX20 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Gemin 3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DDX20

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Alternative Name: DEAD-box helicase 20

Background:

Synonyms: DEAD-box helicase 20 , DP103 , GEMIN3

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq]

Molecular Weight: 92 kDa

Gene ID: 11218

UniProt: Q9UHI6

Pathways: Ribonucleoprotein Complex Subunit Organization