DDX4 antibody (C-Term)

Catalog No. ABIN7468227

This Rabbit Polyclonal antibody specifically detects DDX4 in WB, IHC (p), IF, ICC and IHC (wm). It exhibits reactivity toward Human.

Quick Overview for DDX4 antibody (C-Term) (ABIN7468227)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-DDX4 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Fish, Human, Mouse, Sturgeon (Acipenser)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.54 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DEAD-box helicase 4

Background:

Synonyms: DEAD-box helicase 4 , VASA

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]

Molecular Weight: 79 kDa

Gene ID: 54514

UniProt: Q9NQI0