DDX4 antibody

Catalog No. ABIN969084

Product Details anti-DDX4 Antibody

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purification: purified

Immunogen: Purified recombinant fragment of human DDX4 expressed in E. coli.

Clone: 2F9H5

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-DDX4 antibody (ABIN969084)

Shibata, Tsunekawa, Okamoto-Ito, Akasu, Tokumasu, Noce: "Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein." in: Molecular reproduction and development, Vol. 67, Issue 1, pp. 1-7, (2003) (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Zeeman, Stoop, Boter, Gillis, Castrillon, Oosterhuis, Looijenga: "VASA is a specific marker for both normal and malignant human germ cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 2, pp. 159-66, (2002) (PubMed).

Castrillon, Quade, Wang, Quigley, Crum: "The human VASA gene is specifically expressed in the germ cell lineage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9585-90, (2000) (PubMed).

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4 (DDX4 Products)

Synonyms: VASA antibody, AV206478 antibody, Mvh antibody, RVLG antibody, vasa antibody, vlg1 antibody, DEAD-box helicase 4 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antibody, DEAD-box helicase 4 L homeolog antibody, DDX4 antibody, Ddx4 antibody, ddx4.L antibody

Background:

Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Aliases: VASA,MGC111074,DDX4

Molecular Weight: 76 kDa

Gene ID: 54514

HGNC: 54514