GTF2A1 antibody

Catalog No. ABIN7468367

The Rabbit Polyclonal anti-GTF2A1 antibody (ABIN7468367) specifically detects GTF2A1 in WB, ICC, IHC (p) and IF. The antibody is reactive with Human samples.

Quick Overview for GTF2A1 antibody (ABIN7468367)

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-GTF2A1 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GTF2A1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GTF2A1

Target: GTF2A1 (General Transcription Factor IIA, 1, 19/37kDa (GTF2A1))

Alternative Name: general transcription factor IIA subunit 1

Background:

Synonyms: general transcription factor IIA subunit 1 , TF2A1 , TFIIA , TFIIA-42 , TFIIAL

Background: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A, MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM]

Molecular Weight: 42 kDa

Gene ID: 2957

UniProt: P52655