ATRX antibody (C-Term)

Catalog No. ABIN7468429

The Mouse Monoclonal anti-ATRX antibody has been validated for WB, IF and ICC. It is suitable to detect ATRX in samples from Human.

Quick Overview for ATRX antibody (C-Term) (ABIN7468429)

Target: ATRX (helicase 2, X-linked (ATRX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATRX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT4212

Product Details anti-ATRX Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , C8D30 , BCL-1 , 293T Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX chromatin remodeler

Background:

Synonyms: ATRX chromatin remodeler , JMS , MRX52 , RAD54 , RAD54L , XH2 , XNP , ZNF-HX

Background: The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Molecular Weight: 283 kDa

Gene ID: 546

UniProt: P46100