ITGAL antibody (N-Term)

Catalog No. ABIN7468486

The Rabbit Polyclonal anti-ITGAL antibody has been validated for WB, IF and ICC. It is suitable to detect ITGAL in samples from Human.

Quick Overview for ITGAL antibody (N-Term) (ABIN7468486)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ITGAL Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human CD11a. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: THP-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: integrin subunit alpha L

Background:

Synonyms: integrin subunit alpha L , CD11A , LFA-1 , LFA1A

Background: ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 129 kDa

Gene ID: 3683

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex