ISCU antibody

Catalog No. ABIN7468499

The Rabbit Polyclonal anti-ISCU antibody (ABIN7468499) specifically detects ISCU in WB, IF, ICC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ISCU antibody (ABIN7468499)

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISCU antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ISCU Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ISCU. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS , ISCU-transfected 293T Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.66 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ISCU

Target: ISCU (Iron-sulfur cluster assembly enzyme ISCU, mitochondrial (ISCU))

Alternative Name: iron-sulfur cluster assembly enzyme

Background:

Synonyms: iron-sulfur cluster assembly enzyme , 2310020H20Rik , HML , ISU2 , NIFU , NIFUN , hnifU

Background: Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq]

Molecular Weight: 18 kDa

Gene ID: 23479

UniProt: Q9H1K1