MYH10 antibody (N-Term)

Catalog No. ABIN7468620

This Mouse Monoclonal antibody specifically detects MYH10 in WB, ICC, IF, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for MYH10 antibody (N-Term) (ABIN7468620)

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYH10 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT246

Product Details anti-MYH10 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , MYH10-transfected 293T , NIH-3T3 , mouse brain Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MYH10

Target: MYH10 (Myosin, Heavy Polypeptide 10, Non-Muscle (MYH10))

Alternative Name: myosin heavy chain 10

Background:

Synonyms: myosin heavy chain 10 , NMMHC-IIB , NMMHCB

Background: This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin, it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Molecular Weight: 229 kDa

Gene ID: 4628

UniProt: P35580