NDC80 antibody (AA 56-642)

Catalog No. ABIN7468638

This Mouse Monoclonal antibody specifically detects NDC80 in WB, IF, IP, ICC, FACS, IHC (fro) and PLA. It exhibits reactivity toward Human.

Quick Overview for NDC80 antibody (AA 56-642) (ABIN7468638)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Proximity Ligation Assay (PLA)

Clone: 9G3-23

Product Details anti-NDC80 Antibody

Binding Specificity: AA 56-642

Cross-Reactivity: Hamster, Human, Kangaroo rat (Dipodomys sp.), Mouse

Purification: Affinity purified by Protein A.

Immunogen: Human HEC1 protein consisting of amino acids 56-642.

Isotype: IgG2a

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 kinetochore complex component

Background:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Molecular Weight: 74 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location