NDC80 antibody (pSer165)

Catalog No. ABIN7468641

This Rabbit Polyclonal antibody specifically detects NDC80 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for NDC80 antibody (pSer165) (ABIN7468641)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NDC80 Antibody

Binding Specificity: pSer165

Cross-Reactivity: Human, Kangaroo rat (Dipodomys sp.), Mouse, Rat

Purification: Affinity purified using antigen

Immunogen: Synthetic phosphopeptide corresponding to the amino acids around Ser 165 conjugated to KLH

Isotype: IgG

Application Details

Application Notes: Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Comment:

Positive Control: HeLa , Blocking peptide (GTX70013-PEP) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.127 mg/mL

Buffer: 1X PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 kinetochore complex component

Background:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Molecular Weight: 74 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location