NDC80 antibody (Ser76, Ser77)

Catalog No. ABIN7468642

The Rabbit Polyclonal anti-NDC80 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect NDC80 in samples from Human.

Quick Overview for NDC80 antibody (Ser76, Ser77) (ABIN7468642)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NDC80 Antibody

Binding Specificity: Ser76, Ser77

Cross-Reactivity: Human

Purification: Affinity purified using antigen

Immunogen: Synthetic peptide corresponding to the amino acids around Ser 76/77 conjugated to KLH

Isotype: IgG

Application Details

Application Notes: Recommended Starting Dilutions:
For WB: Use at a dilution of 1:400
For ICC/IF: Use at a dilution of 1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Comment:

Positive Control: HeLa , Blocking peptide (GTX70014-PEP)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.816 mg/mL

Buffer: 1X PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 kinetochore complex component

Background:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Molecular Weight: 74 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location