NDC80 antibody (AA 30-80)

Catalog No. ABIN7468644

This Rabbit Polyclonal antibody specifically detects NDC80 in WB, ELISA, IF, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for NDC80 antibody (AA 30-80) (ABIN7468644)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NDC80 Antibody

Binding Specificity: AA 30-80

Cross-Reactivity: Human, Mouse, Rat

Purification: Affinity purified using antigen

Immunogen: A Synthetic peptide within a.a. 30~80 around Ser55

Isotype: IgG

Application Details

Application Notes: Recommended Starting Dilutions:
For WB: Use at a dilution of 1:750
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Comment:

Positive Control: HeLa , Blocking peptide (GTX70016-PEP)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1X PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 kinetochore complex component

Background:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Molecular Weight: 74 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location