Protein A antibody (HRP)

Catalog No. ABIN7468794

This Chicken Polyclonal antibody specifically detects Protein A in ELISA and WB. It exhibits reactivity toward Chicken.

Quick Overview for Protein A antibody (HRP) (ABIN7468794)

Target: Protein A

Reactivity: Chicken

Host: Chicken

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB)

Product Details anti-Protein A Antibody (HRP)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freund's adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP).

Isotype: IgY

Application Details

Application Notes: For ELISA: Use at a dilution of 1:5,000-1:20,000. For WB: Use at a dilution of 1:1,000-1:20,000. Optimal dilutions/concentrations should be determined by the researcher.

Comment:

Positive Control: Protein A

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.43 mg/mL

Buffer: 0.05M Tris ( pH 7.4), 0.15M NaCl, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Protein A

Target: Protein A