PUM2 antibody (C-Term)

Catalog No. ABIN7468827

This Rabbit Polyclonal antibody specifically detects PUM2 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PUM2 antibody (C-Term) (ABIN7468827)

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PUM2 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Pumilio 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Human ESC , mouse ovary

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PUM2

Target: PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))

Alternative Name: pumilio RNA binding family member 2

Background:

Synonyms: pumilio RNA binding family member 2 , PUMH2 , PUML2

Background: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

Molecular Weight: 114 kDa

Gene ID: 23369

UniProt: Q8TB72

Pathways: Ribonucleoprotein Complex Subunit Organization