PKLR antibody

Catalog No. ABIN7468839

This Rabbit Polyclonal antibody specifically detects PKLR in WB. It exhibits reactivity toward Human.

Quick Overview for PKLR antibody (ABIN7468839)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKLR Antibody

Specificity: This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562 , Raw264.7 , rat liver , human PKLR-transfected 293T cells Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.62 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: pyruvate kinase L/R

Background:

Synonyms: pyruvate kinase L/R , PK1 , PKL , PKRL , RPK

Background: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 62 kDa

Gene ID: 5313

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process