PKLR antibody (N-Term)

Catalog No. ABIN2776945

The Rabbit Polyclonal anti-PKLR antibody is suitable to detect PKLR in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for PKLR antibody (N-Term) (ABIN2776945)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PKLR Antibody

Binding Specificity: N-Term

Purpose: PKLR Antibody - N-terminal region

Sequence: STSIIATIGP ASRSVERLKE MIKAGMNIAR LNFSHGSHEY HAESIANVRE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PKLR. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PKLR

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8109754

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR

Background:

Background Information: PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Gene Name: Pyruvate kinase, liver and RBC

Alternative Symbols: PK1, PKL, RPK, PKRL

Protein Name: Pyruvate kinase isozymes R/L

Molecular Weight: 58kDa

Gene ID: 5313

NCBI Accession: NP_870986

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process