14-3-3 gamma antibody

Catalog No. ABIN7469224

The Rabbit Polyclonal anti-14-3-3 gamma antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect 14-3-3 gamma in samples from Human.

Quick Overview for 14-3-3 gamma antibody (ABIN7469224)

Target: 14-3-3 gamma (YWHAG1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-14-3-3 gamma Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , HepG2 , K562 , THP-1 , HL-60 Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.24 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Alternative Name: 14-3-3 gamma

Background:

Synonyms: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma , 14-3-3GAMMA , EIEE56 , PPP1R170

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

Molecular Weight: 28 kDa

Gene ID: 7532

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase