MBD5 antibody

Catalog No. ABIN7469295

This Rabbit Polyclonal antibody specifically detects MBD5 in WB. It exhibits reactivity toward Human.

Quick Overview for MBD5 antibody (ABIN7469295)

Target: MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MBD5 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MBD5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2 nuclear extract Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.19 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MBD5

Target: MBD5 (Methyl-CpG Binding Domain Protein 5 (MBD5))

Alternative Name: MBD5

Background:

Synonyms: methyl-CpG binding domain protein 5 , MRD1

Background: This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause mental retardation autosomal dominant type 1. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq]

Molecular Weight: 160 kDa

Gene ID: 55777

UniProt: Q9P267

Pathways: Chromatin Binding