MafF antibody

Catalog No. ABIN7469478

This Rabbit Polyclonal antibody specifically detects MafF in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for MafF antibody (ABIN7469478)

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MafF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MafF Antibody

Cross-Reactivity: Human, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAFF. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Huh7 , HepG2 , human MAFF-transfected 293T , A431 nuclear extract , rat skin Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MafF

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Alternative Name: MAFF

Background:

Synonyms: MAF bZIP transcription factor F , U-MAF , hMafF

Background: The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 18 kDa

Gene ID: 23764

UniProt: Q9ULX9

Pathways: Myometrial Relaxation and Contraction