MSH3 antibody (C-Term)

Catalog No. ABIN7469495

This anti-MSH3 antibody is a Rabbit Polyclonal antibody detecting MSH3 in WB, IF, IHC (p) and ICC. Suitable for Human.

Quick Overview for MSH3 antibody (C-Term) (ABIN7469495)

Target: MSH3 (MutS Homolog 3 (MSH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-MSH3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MSH3

Target: MSH3 (MutS Homolog 3 (MSH3))

Alternative Name: MSH3

Background:

Synonyms: mutS homolog 3 , DUP , FAP4 , MRP1

Background: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Molecular Weight: 127 kDa

Gene ID: 4437

UniProt: P20585

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response