Rubicon antibody (C-Term)

Catalog No. ABIN7469583

This Rabbit Polyclonal antibody specifically detects Rubicon in WB and IP. It exhibits reactivity toward Human.

Quick Overview for Rubicon antibody (C-Term) (ABIN7469583)

Target: Rubicon (KIAA0226) (KIAA0226)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rubicon antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Rubicon Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Rubicon. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-MB-231 , NT2D1 , human Rubicon-transfected 293T cells Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Rubicon

Target: Rubicon (KIAA0226) (KIAA0226)

Alternative Name: Rubicon

Background:

Synonyms: rubicon autophagy regulator , KIAA0226 , RUBICON , SCAR15

Background: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.

Molecular Weight: 109 kDa

Gene ID: 9711

UniProt: Q92622

Pathways: Autophagy