SART3 antibody (C-Term)

Catalog No. ABIN7469611

The Rabbit Polyclonal anti-SART3 antibody has been validated for WB, IF, IP, IHC (p) and ICC. It is suitable to detect SART3 in samples from Human.

Quick Overview for SART3 antibody (C-Term) (ABIN7469611)

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-SART3 Antibody

Binding Specificity: C-Term

Specificity: Knockdown/Knockout validation was supported by references (PMID:26990993)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , H1299 , HeLa , HepG2 Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3

Background:

Synonyms: spliceosome associated factor 3, U4/U6 recycling protein , DSAP1 , P100 , RP11-13G14 , TIP110 , p110 , p110(nrb)

Background: The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq]

Molecular Weight: 110 kDa

Gene ID: 9733

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization