Recombinant ACE2 antibody (AA 705-805)

Catalog No. ABIN7477373

The Rabbit Monoclonal anti-ACE2 antibody has been validated for IHC. It is suitable to detect ACE2 in samples from Human.

Quick Overview for Recombinant ACE2 antibody (AA 705-805) (ABIN7477373)

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ACE2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-919R

Product Details anti-ACE2 Antibody

Binding Specificity: AA 705-805

Immunogen: Recombinant fragment (around aa705-805) of human ACE2 (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: Cells of proximal tubuli should show a moderate to strong ACE2 immunostaining with accentuation of the staining at the apical membrane.

Negative Control: Kidney: Cells of distal tubuli and glomeruli should not show ACE2 immunostaining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2

Background: Angiotensin I converting enzyme 2,ACE2 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q9BYF1

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour