Recombinant Caldesmon antibody

Catalog No. ABIN7477385

The Rabbit Monoclonal anti-Caldesmon antibody has been validated for IHC. It is suitable to detect Caldesmon in samples from Human.

Quick Overview for Recombinant Caldesmon antibody (ABIN7477385)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-538R

Product Details anti-Caldesmon Antibody

Immunogen: Recombinant human full-length CALD1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong caldesmon staining should be seen of the muscularis mucosa and of a membrane-like layer along the basement membranes of crypts (intestinal subepithelial myofibroblasts).

Negative Control: Colon: Normal epithelial cells must not show caldesmon staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction