Recombinant CNN1 antibody (C-Term)

Catalog No. ABIN7477387

This anti-CNN1 antibody is a Rabbit Monoclonal antibody detecting CNN1 in IHC. Suitable for Human.

Quick Overview for Recombinant CNN1 antibody (C-Term) (ABIN7477387)

Target: CNN1 (Calponin 1 (CNN1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CNN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-455R

Product Details anti-CNN1 Antibody

Binding Specificity: C-Term

Immunogen: Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels.

Negative Control: Colon: Calponin 1 staining should be absent in all epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CNN1

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: Calponin 1

Background: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P51911