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Recombinant CD3 antibody (AA 20-40)

The Rabbit Monoclonal anti-CD3 antibody has been validated for IHC. It is suitable to detect CD3 in samples from Human.

Appendix mucosa In the appendix numerous CD3 positive T lymphocytes occur in the epithelium the lamina propria and in germinal centres (Recombinant CD3 antibody (AA 20-40))

Squamous cell carcinoma containing numerous CD3 positive T lymphocytes (Recombinant CD3 antibody (AA 20-40))

Diffuse large B cell lymphoma infiltrated by numerous CD3 positive non neoplastic lymphocytes (Recombinant CD3 antibody (AA 20-40))

3 images

Catalog No. ABIN7477391

Quick Overview for Recombinant CD3 antibody (AA 20-40) (ABIN7477391)

Target

See all CD3 Antibodies

CD3

Antibody Type

Recombinant Antibody

Reactivity

187
99
27
20
17
10
8
8
7
7
5
3
2
2
1

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This CD3 antibody is un-conjugated

Application

291
83
56
53
46
37
33
22
11
8
7
6
4
3
2
2
2
2
2
2
2
2
1
1
1

Immunohistochemistry (IHC)

Clone

MSVA-003R

Discover our top product CD3 Primary Antibody

Binding Specificity
3

2

1

1

1
AA 20-40

Immunogen

A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)

Isotype

IgG
anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Mouse, Rat IHC, IF, WB Host: Rabbit Polyclonal unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), ICC, MS Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, MS Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, Func, MS Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody
CD3 Reactivity: Human FACS, IP, MS Host: Mouse Monoclonal MEM-57 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).
Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.

Protocol

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Target

CD3

Alternative Name

CD3

Background

CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

P07766

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway

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