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Recombinant CD5 antibody (AA 32-372)

This Rabbit Monoclonal antibody specifically detects CD5 in IHC. It exhibits reactivity toward Human.

Lymph node B CLL with strong CD5 positivity of all tumor cells. (Recombinant CD5 antibody (AA 32-372))

Tonsil Strong CD5 positivity of T lymphocytes interfollicular and in germinal centres (Recombinant CD5 antibody (AA 32-372))

Strong CD5 immunostaining of T lymphocytes in the ileum mucosa (Recombinant CD5 antibody (AA 32-372))

3 images

Catalog No. ABIN7477395

Quick Overview for Recombinant CD5 antibody (AA 32-372) (ABIN7477395)

Target

See all CD5 Antibodies

CD5

Antibody Type

Recombinant Antibody

Reactivity

263
80
61
29
24
20
16
13
12
12
12
12
9
2
1

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This CD5 antibody is un-conjugated

Application

269
121
96
88
71
48
29
29
21
21
21
18
13
13
6
4
3
3
2
2
2
1
1

Immunohistochemistry (IHC)

Clone

MSVA-005R

Discover our top product CD5 Primary Antibody

Binding Specificity
23

16

15

8

5

4

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 32-372

Immunogen

Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).

Isotype

IgG
anti-CD5 (CD5) (AA 105-210) antibody
CD5 Reactivity: Human WB Host: Goat Polyclonal unconjugated

anti-CD5 (CD5) (AA 269-366) antibody
CD5 Reactivity: Human FACS, WB, IHC, IF, StM Host: Mouse Monoclonal CD5-2416 unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IHC, IP, ICC Host: Mouse Monoclonal L17F12 unconjugated

anti-CD5 (CD5) (AA 25-372) antibody
CD5 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD5 (CD5) (AA 403-495) antibody
CD5 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 6C3B2 unconjugated

anti-CD5 (CD5) (AA 27-233) antibody
CD5 Reactivity: Human FACS, WB, ELISA Host: Mouse Monoclonal 6A11 unconjugated

anti-CD5 (CD5) (AA 269-366) antibody
CD5 Reactivity: Human FACS, IHC, IF, StM Host: Mouse Monoclonal CD5-2418 unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (p) Host: Mouse Monoclonal MEM-32 unconjugated

anti-CD5 (CD5) antibody
CD5 Reactivity: Human FACS, WB, IP, ELISA, IHC (fro) Host: Mouse Monoclonal CRIS1 unconjugated

anti-CD5 (CD5) antibody (PE)
CD5 Reactivity: Human FACS Host: Mouse Monoclonal L17F12 PE

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen.
Negative Control: Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive).

Protocol

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Target

CD5

Alternative Name

CD5

Background

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

P06127

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