FCER2 antibody (AA 48-321)
Quick Overview for FCER2 antibody (AA 48-321) (ABIN7477405)
Target
See all FCER2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 48-321
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Immunogen
- Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Tonsil: The follicular dendritic cells of the germinal centres should show a strong CD23 immunostaining while the majority of lymphocytes in the mantle zone of the follicles should show an at least weak staining.
Negative Control: Tonsil: The squamous epithelial cells and T-cells in the interfollicular T-zones should not show any CD23 immunostaining.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
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Alternative Name
- CD23
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Background
- BLAST-2, C-type lectin domain family 4, member J, Fc fragment of IgE low affinity II receptor, Fc-epsilon-RII, FCER2, FCER2A, FceRII, IgE-binding factor (IGEBF), Immunoglobulin E receptor, low affinity II, Lymphocyte IgE receptor,CD23 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P06734
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target
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