Recombinant CD34 antibody

Catalog No. ABIN7477411

This anti-CD34 antibody is a Rabbit Monoclonal antibody detecting CD34 in IHC. Suitable for Human.

Quick Overview for Recombinant CD34 antibody (ABIN7477411)

Target: CD34

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-034R

Product Details anti-CD34 Antibody

Immunogen: Recombinant full-length human HPCA1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level.

Negative Control: Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P28906