CD163 antibody (AA 43-196)

Catalog No. ABIN7477417

This anti-CD163 antibody is a Mouse Monoclonal antibody detecting CD163 in IHC. Suitable for Human.

Quick Overview for CD163 antibody (AA 43-196) (ABIN7477417)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-163M

Product Details anti-CD163 Antibody

Binding Specificity: AA 43-196

Immunogen: Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.

Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues