CD163 antibody (AA 43-196)

Catalog No. ABIN7470589

The Mouse Monoclonal anti-CD163 antibody (Clone 163M) (ABIN7470589) specifically detects CD163 in IHC. The antibody is reactive with Human samples.

Quick Overview for CD163 antibody (AA 43-196) (ABIN7470589)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 163M

Product Details anti-CD163 Antibody

Binding Specificity: AA 43-196

Purpose: CD163 antibody (163M)

Immunogen: Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.

Product Description: CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: Gene Alias: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M132