Recombinant CTLA4 antibody

Catalog No. ABIN7477429

This anti-CTLA4 antibody is a Rabbit Monoclonal antibody detecting CTLA4 in IHC. Suitable for Human.

Quick Overview for Recombinant CTLA4 antibody (ABIN7477429)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-152R

Product Details anti-CTLA4 Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.

Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12),CTLA-4 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P16410

Pathways: Cancer Immune Checkpoints