CSTA antibody

Catalog No. ABIN7477431

This Mouse Monoclonal antibody specifically detects CSTA in IHC. It exhibits reactivity toward Human.

Quick Overview for CSTA antibody (ABIN7477431)

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSTA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-461M

Product Details anti-CSTA Antibody

Immunogen: Recombinant full-length human CSTA protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Skin: A strong nuclear and cytoplasmic Cystatin A immunostaining should be seen in the granular layer.

Negative Control: Colon: No epithelial cell staining should be seen. Few inflammatory cells may show Cystatin A positivity.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CSTA

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Alternative Name: Cystatin A

Background: STF1, STFA, AREI, cstA, Stefin A,Cystatin A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P01040

Pathways: Response to Water Deprivation