Recombinant Cytokeratin 18 antibody
Quick Overview for Recombinant Cytokeratin 18 antibody (ABIN7477447)
Target
See all Cytokeratin 18 (KRT18) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Immunogen
- Recombinant human full-length KRT18 protein
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:100-1:200
-
Comment
-
Positive Control: Liver: At least a weak to moderate, predominantly membranous staining should be seen in virtually all hepatocytes. Appendix: A strong staining should be seen in all epithelial cells.
Negative Control: Appendix: Staining should be absent in all non-epithelial cells of the appendix.
-
Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
-
Alternative Name
- Cytokeratin 18
-
Background
- Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 18 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P05783
-
Pathways
- Apoptosis, Caspase Cascade in Apoptosis
Target
-