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Recombinant Cytokeratin 18 antibody

This anti-Cytokeratin 18 antibody is a Rabbit Monoclonal antibody detecting Cytokeratin 18 in IHC. Suitable for Human.
Catalog No. ABIN7477447

Quick Overview for Recombinant Cytokeratin 18 antibody (ABIN7477447)

Target

See all Cytokeratin 18 (KRT18) Antibodies
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Antibody Type

Recombinant Antibody

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This Cytokeratin 18 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

MSVA-118R
  • Immunogen

    Recombinant human full-length KRT18 protein

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Liver: At least a weak to moderate, predominantly membranous staining should be seen in virtually all hepatocytes. Appendix: A strong staining should be seen in all epithelial cells.

    Negative Control: Appendix: Staining should be absent in all non-epithelial cells of the appendix.

    Protocol

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    Alternative Name

    Cytokeratin 18

    Background

    Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 18 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P05783

    Pathways

    Apoptosis, Caspase Cascade in Apoptosis
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