Cytokeratin 19 antibody
Quick Overview for Cytokeratin 19 antibody (ABIN7470605)
Target
See all Cytokeratin 19 (KRT19) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Cytokeratin 19 antibody (119M)
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Immunogen
- Recombinant human KRT19 protein
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Isotype
- IgG1, IgG2a, kappa, lambda
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Colon: A strong immunostaining is seen in all epithelial cells. Tonsil: A moderate to strong immunostaining occurs in the basal cell layer of the squamous epithelium of the tonsil surface.
Product Description: Cytokeratin 19 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: All non-epithelial cells are CK19 negative. Tonsil: All non-basal squamous epithelial cells and all non-epithelial cells are CK19 negative.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Expiry Date
- 24 months
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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Alternative Name
- Cytokeratin 19
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Background
- Gene Alias: k19, k1cs, Keratin 19 Keratin Type i 40kD, krt21
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UniProt
- P08727
Target
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