Cytokeratin 19 antibody

Catalog No. ABIN7477449

This Mouse Monoclonal antibody specifically detects Cytokeratin 19 in IHC. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 19 antibody (ABIN7477449)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-119M

Product Details anti-Cytokeratin 19 Antibody

Immunogen: Recombinant human KRT19 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong immunostaining is seen in all epithelial cells. Tonsil: A moderate to strong immunostaining occurs in the basal cell layer of the squamous epithelium of the tonsil surface.

Negative Control: Colon: All non-epithelial cells are CK19 negative. Tonsil: All non-basal squamous epithelial cells and all non-epithelial cells are CK19 negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19

Background: K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19,Cytokeratin 19 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08727