Recombinant pan Keratin antibody

Catalog No. ABIN7477453

This anti-pan Keratin antibody is a Rabbit Monoclonal antibody detecting pan Keratin in IHC. Suitable for Human.

Quick Overview for Recombinant pan Keratin antibody (ABIN7477453)

Target: pan Keratin (panKRT)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-000R

Product Details anti-pan Keratin Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A strong staining of all bile duct epithelial cells and an at least moderate, predominantly membranous staining of most hepatocytes should be seen.

Negative Control: Tonsil: All lymphocytes must not stain. However, interstitial reticulum cells with dendritic/reticular pattern show a weak to moderate fibrillar staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Cytokeratin, Pan

Background: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, Pan antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q7Z794, Q01546