Recombinant Decorin antibody (AA 212-336)
Quick Overview for Recombinant Decorin antibody (AA 212-336) (ABIN7477455)
Target
See all Decorin (DCN) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 212-336
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Immunogen
- Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Fallopian tube: A strong staining should be seen in the stroma while epithelial cells remain negative.
Negative Control: Fallopian tube: Epithelial cells should not show any staining.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Decorin (DCN)
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Alternative Name
- Decorin
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Background
- Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B,Decorin antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07585
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Pathways
- Glycosaminoglycan Metabolic Process
Target
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