Recombinant Elastin antibody (full length)
Quick Overview for Recombinant Elastin antibody (full length) (ABIN7477467)
Target
See all Elastin (ELN) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- full length
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Purpose
- Elastin antibody (648R)
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Immunogen
- Recombinant full-length human Elastin protein
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).
Product Description/Background: Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Expiry Date
- 24 months
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- Elastin (ELN)
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Alternative Name
- Elastin
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Background
- Gene Alias: Elastin, ELN, SVAS, Tropoelastin, WBS, WS
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UniProt
- P15502
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Pathways
- Regulation of Actin Filament Polymerization
Target
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