F13A1 antibody (AA 46-181)

Catalog No. ABIN7477475

The Mouse Monoclonal anti-F13A1 antibody has been validated for IHC. It is suitable to detect F13A1 in samples from Human.

Quick Overview for F13A1 antibody (AA 46-181) (ABIN7477475)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F13A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-813R

Product Details anti-F13A1 Antibody

Binding Specificity: AA 46-181

Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining.

Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Factor XIII alpha

Background: Coagulation factor XIII A chain, Coagulation factor XIII A1 polypeptide, F13A, F13a1, Fibrin stabilizing factor, A subunit, Fibrinoligase, FSF, A subunit, Protein-glutamine gamma-glutamyltransferase A chain, TGase, Transglutaminase. Plasma,Factor XIII alpha antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P00488