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GATA3 antibody (full length)

This Mouse Monoclonal antibody specifically detects GATA3 in IHC. It exhibits reactivity toward Human.
Catalog No. ABIN7477479

Quick Overview for GATA3 antibody (full length) (ABIN7477479)

Target

See all GATA3 Antibodies
GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This GATA3 antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

450M
  • Binding Specificity

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    full length

    Purpose

    GATA3 antibody (450M)

    Immunogen

    Recombinant full-length human GATA-3 protein

    Isotype

    IgG2b, kappa
  • Application Notes

    IHC 1:50-1:100

    Comment

    Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.

    Product Description/Background: GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.

    Protocol

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

    Expiry Date

    24 months
  • Target

    GATA3 (GATA Binding Protein 3 (GATA3))

    Alternative Name

    GATA3

    Background

    Gene Alias: GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-5

    UniProt

    P23771

    Pathways

    Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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