Recombinant IgD antibody

Catalog No. ABIN7477499

The Rabbit Monoclonal anti-IgD antibody has been validated for IHC. It is suitable to detect IgD in samples from Human.

Quick Overview for Recombinant IgD antibody (ABIN7477499)

Target: IgD

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IgD antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-701R

Product Details anti-IgD Antibody

Immunogen: Recombinant human IGHD fragment

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong IgD staining should be seen in lymphocytes of the mantle zone.

Negative Control: Tonsil: Epithelial cells and most ""non-mantle zone"" lymphocytes should not stain for IgD.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for IgD

Target: IgD

Target Type: Antibody

Background: Pivotal molecule in the immune response,IgD antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P01880